The Ameliorative Effect of Echinacea Purpurea Against Gamma Radiation Induced Oxidative Stress and Immune Responses in Male Rats
نویسنده
چکیده
While radiation hazards, due to free radical generation, present an enormous challenge for biological and medical safety, Echinacea purpurea is a potent scavenger of a variety of free radicals. The aim of this study was to investigate the radioprotective effect of Echinacea purpurea against oxidative stress in spleen tissues and alterations in some immune responses. Rats were whole body irradiated with 3Gy/week up to a total dose of 6Gy. E. purpurea (30mg/Kg body weight/day) was given to rats, via gavages, during 14 days before irradiation and 7 days after each radiation dose. Animals were sacrificed on the 7 day after the last radiation dose. A significant increase in xanthine oxidase (XO) activity along with a significant decrease in xanthine dehydrogenase (XDH) activity was recorded in the spleen of irradiated rats. In addition, the levels of oxidative biomarkers; thiobarbituric acid reactive substances (TBARS), protein carbonyl and advanced oxidation protein products (AOPP) showed a significant increase. The activity of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) showed significant increases, while glutathione peroxidase (GSHPx) activity showed a significant decrease and glutathione content showed normal value in the spleen of irradiated rats. Irradiation resulted also in significant decreases in total white blood cells (WBCs) count, lymphocytes, monocytes, neutrophils, eosinophils, basophils and potassium level. On the other hand, a significant increase in the level of sodium and C-reactive protein (CRP) was recorded in the serum of irradiated rats. E. purpurea supplementation has significantly improved oxidative stress in the spleen of irradiated rats. Total and differential white blood cells (WBCs) count showed a significant increase compared to their corresponding values in irradiated rats while serum CRP returned normal. It could be concluded that E. purpurea may afford protection against radiation-induced oxidative stress in spleen tissues and is involved in the modulation of immune responses.
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